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pisum sativum agglutinin  (Vector Laboratories)


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    Structured Review

    Vector Laboratories pisum sativum agglutinin
    Pisum Sativum Agglutinin, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 14 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/B-1055-5/Biotinylated+Pisum+Sativum+Agglutinin+(PSA)/pm40825516-93-15-20
    Average 93 stars, based on 14 article reviews
    pisum sativum agglutinin - by Bioz Stars, 2026-11
    93/100 stars

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    Related Articles

    Saline:

    Article Title: Biophysical principles of liquid–liquid phase separation
    Article Snippet: ll OPEN ACCESS iScience

    Incubation:

    Article Title: Biophysical principles of liquid–liquid phase separation
    Article Snippet: ll OPEN ACCESS iScience

    Article Title: Perturbation of placental protein glycosylation by endoplasmic reticulum stress promotes maladaptation of maternal hepatic glucose metabolism
    Article Snippet: Resin was removed with saturated sodium ethoxide diluted 1: 1 with absolute ethanol for 12 min, followed by washes in ethanol and distilled water, then endogenous peroxidase was quenched for 8 min with 10% aqueous hydrogen peroxide before exposure to 0.03% trypsin from porcine pancreas (T7409, Type II-S; Sigma-Aldrich) in 50 mM Tris-buffered saline (TBS), pH 7.6, with added 1% CaCl 2 for 4 min at 37°C. .. After washing in water, sections were incubated in 10 μm/mL biotinylated lectin (Concanavalin A, P. sativum and D. stramonium Agglutinins (B-1005, B-1055, B-1185) Vector Laboratories, Peterborough, UK) in 50 mM TBS containing 1 mM calcium chloride for 75 min at 37°C, washed 3 times 5 min in the same buffer, then treated with 5 μg/mL avidin peroxidase (A3151, Sigma-Aldrich) in 125 mM TBS, pH 7.6, with added 347 mM sodium chloride for 90 min at 37°C. .. Sections were washed in 50 mM TBS 3 times 5min and sites of lectin binding revealed with 0.05% (w/v) 3′3′diaminobenzidine tetrahydrochloride dihydrate (26,189-0, Sigma-Aldrich) in 50 mM TBS, pH 7.6, and 0.015% hydrogen peroxide (100 vol) for 7 min at 18°C.

    Avidin-Biotin Assay:

    Article Title: Biophysical principles of liquid–liquid phase separation
    Article Snippet: ll OPEN ACCESS iScience

    Article Title: Perturbation of placental protein glycosylation by endoplasmic reticulum stress promotes maladaptation of maternal hepatic glucose metabolism
    Article Snippet: Resin was removed with saturated sodium ethoxide diluted 1: 1 with absolute ethanol for 12 min, followed by washes in ethanol and distilled water, then endogenous peroxidase was quenched for 8 min with 10% aqueous hydrogen peroxide before exposure to 0.03% trypsin from porcine pancreas (T7409, Type II-S; Sigma-Aldrich) in 50 mM Tris-buffered saline (TBS), pH 7.6, with added 1% CaCl 2 for 4 min at 37°C. .. After washing in water, sections were incubated in 10 μm/mL biotinylated lectin (Concanavalin A, P. sativum and D. stramonium Agglutinins (B-1005, B-1055, B-1185) Vector Laboratories, Peterborough, UK) in 50 mM TBS containing 1 mM calcium chloride for 75 min at 37°C, washed 3 times 5 min in the same buffer, then treated with 5 μg/mL avidin peroxidase (A3151, Sigma-Aldrich) in 125 mM TBS, pH 7.6, with added 347 mM sodium chloride for 90 min at 37°C. .. Sections were washed in 50 mM TBS 3 times 5min and sites of lectin binding revealed with 0.05% (w/v) 3′3′diaminobenzidine tetrahydrochloride dihydrate (26,189-0, Sigma-Aldrich) in 50 mM TBS, pH 7.6, and 0.015% hydrogen peroxide (100 vol) for 7 min at 18°C.

    Binding Assay:

    Article Title: Biophysical principles of liquid–liquid phase separation
    Article Snippet: ll OPEN ACCESS iScience

    other:

    Article Title: N-terminome analyses underscore the prevalence of SPPL3-mediated intramembrane proteolysis among Golgi-resident enzymes and its role in Golgi enzyme secretion.
    Article Snippet: Cells were washed with staining buffer (PBS supplemented with 2% (v/v) fetal calf serum, 2 mM EDTA) and were then stained successively with a near-IR fluorescent reactive dye (1:500, Thermo Fisher Scientific), biotin-conjugated lectins and streptavidin-PE (1:1000, Thermo Fisher Scientific) diluted in FACS buffer.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Aberrant glycosylation patterns as potential biomarkers for diagnosis and disease progression in bullous pemphigoid
    Article Snippet: .. Briefly, during Lectin ELISA of serum and blister fluid, biotinylated lectins were diluted with 1% BSA to the following concentrations: HPA-L (Vector, B-1115) 5μg/mL, SNA (Vector, B-1305) 0.5μg/mL, PSA (Vector, B-1055) 0.5μg/mL, SBA (Vector, B-1015) 0.5μg/mL, RCA120 (Vector, B-1085) 0.05μg/mL, AAL (Vector, B-1395) 0.01μg/mL, while during Lectin ELISA of saliva, biotinylated lectins were diluted to the following concentrations: HPA-L 5μg/mL, SNA 2μg/mL, PSA 1μg/mL, SBA 5μg/mL, RCA120 1μg/mL, AAL 1μg/mL. .. Streptavidin linked to HRP (Vector, SA-5014) was diluted 1:2000 and incubated at 37°C for 30 minutes.

    Staining:

    Article Title: Layer- and region-specific distribution of chondroitinase-resistant, lectin-binding extracellular matrix structures in the mouse somatosensory cortex and hippocampus.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.

    Whole Genome Amplification:

    Article Title: Layer- and region-specific distribution of chondroitinase-resistant, lectin-binding extracellular matrix structures in the mouse somatosensory cortex and hippocampus.
    Article Snippet: This is a PDF file of an article that has undergone enhancements after acceptance, such as the addition of a cover page and metadata, and formatting for readability, but it is not yet the definitive version of record.. This version will undergo additional copyediting, typesetting and review before it is published in its final form, but we are providing this version to give early visibility of the article.. Please note that, during the production process, errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.



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    Correlation analysis of serum and blister fluid glycosylation with BP clinical indicators, and diagnostic accuracy assessed by ROC analysis. (a) Evaluation of the correlation between 6 glycosylation structures (binding to PHA-L, SNA, PSA, SBA, RCA120, AAL) and clinical indicators (BP180 and BP230 antibody titers, eosinophil count and percentage, white blood cell count, total serum IgE titer, erythrocyte sedimentation rate, high-sensitivity C-reactive protein level, hospital stay duration, age) in serum. (b) Evaluation of the correlation between 6 glycosylation structures (binding to PHA-L, SNA, PSA, SBA, RCA120, AAL) and clinical indicators (BP180 and BP230 antibody titers, eosinophil count and percentage, total protein, albumin, sodium, potassium, calcium ion concentrations, age, and the six glycosylation modifications) in blister fluid. Spearman’s correlation analysis was used for statistical testing. X indicates p>0.05 after statistical testing. Red color represents positive correlation, while blue color represents negative correlation. The larger and deeper the circle, the stronger the correlation coefficient. (c) ROC analysis for serum glycopatterns recognized by lectin PSA, SNA, SBA to discriminate BP patients from healthy controls. (d) ROC analysis for blister fluid glycopatterns recognized by lectin RCA120, SBA, AAL to discriminate BP patients from control group. The area under the curve (AUC) is used as a metric of diagnostic ability.

    Journal: Frontiers in Immunology

    Article Title: Aberrant glycosylation patterns as potential biomarkers for diagnosis and disease progression in bullous pemphigoid

    doi: 10.3389/fimmu.2025.1538126

    Figure Lengend Snippet: Correlation analysis of serum and blister fluid glycosylation with BP clinical indicators, and diagnostic accuracy assessed by ROC analysis. (a) Evaluation of the correlation between 6 glycosylation structures (binding to PHA-L, SNA, PSA, SBA, RCA120, AAL) and clinical indicators (BP180 and BP230 antibody titers, eosinophil count and percentage, white blood cell count, total serum IgE titer, erythrocyte sedimentation rate, high-sensitivity C-reactive protein level, hospital stay duration, age) in serum. (b) Evaluation of the correlation between 6 glycosylation structures (binding to PHA-L, SNA, PSA, SBA, RCA120, AAL) and clinical indicators (BP180 and BP230 antibody titers, eosinophil count and percentage, total protein, albumin, sodium, potassium, calcium ion concentrations, age, and the six glycosylation modifications) in blister fluid. Spearman’s correlation analysis was used for statistical testing. X indicates p>0.05 after statistical testing. Red color represents positive correlation, while blue color represents negative correlation. The larger and deeper the circle, the stronger the correlation coefficient. (c) ROC analysis for serum glycopatterns recognized by lectin PSA, SNA, SBA to discriminate BP patients from healthy controls. (d) ROC analysis for blister fluid glycopatterns recognized by lectin RCA120, SBA, AAL to discriminate BP patients from control group. The area under the curve (AUC) is used as a metric of diagnostic ability.

    Article Snippet: Briefly, during Lectin ELISA of serum and blister fluid, biotinylated lectins were diluted with 1% BSA to the following concentrations: HPA-L (Vector, B-1115) 5μg/mL, SNA (Vector, B-1305) 0.5μg/mL, PSA (Vector, B-1055) 0.5μg/mL, SBA (Vector, B-1015) 0.5μg/mL, RCA120 (Vector, B-1085) 0.05μg/mL, AAL (Vector, B-1395) 0.01μg/mL, while during Lectin ELISA of saliva, biotinylated lectins were diluted to the following concentrations: HPA-L 5μg/mL, SNA 2μg/mL, PSA 1μg/mL, SBA 5μg/mL, RCA120 1μg/mL, AAL 1μg/mL.

    Techniques: Glycoproteomics, Diagnostic Assay, Binding Assay, Cell Counting, Sedimentation, Control

    Correlation of salivary glycosylation with BP clinical indicators and diagnostic accuracy of selected lectins via ROC analysis. (a) Correlation analysis was conducted to identify the relationship between changes in six glycan structures (specifically binding to PHA-L, SNA, PSA, SBA, RCA120, and AAL) in saliva and disease severity indicators (BP180 antibody titer, BP230 antibody titer, erythema and wheal score, mucosal damage score, BPDAI score). BP patients were categorized into four subgroups based on the positivity of salivary anti-BP180-NC16A and the presence of oral mucosal damage. Spearman’s correlation analysis was used for statistical testing. X indicates p>0.05 after statistical testing. Red color represents positive correlation, while blue color represents negative correlation. The larger and deeper the circle, the stronger the correlation coefficient. The numerical values in the middle represent the Spearman correlation coefficients. (b) Changes in the glycan structures bound to SNA, and AAL in the saliva of BP patients were detected using lectin ELISA before treatment at admission and after significant improvement at discharge (n=11). The Wilcoxon Signed-Rank test was used for statistical analysis. (c) ROC analysis was conducted to assess the glycan structures bound to SNA and AAL in saliva as biomarkers. AUC was used as an indicator to evaluate diagnostic capability.

    Journal: Frontiers in Immunology

    Article Title: Aberrant glycosylation patterns as potential biomarkers for diagnosis and disease progression in bullous pemphigoid

    doi: 10.3389/fimmu.2025.1538126

    Figure Lengend Snippet: Correlation of salivary glycosylation with BP clinical indicators and diagnostic accuracy of selected lectins via ROC analysis. (a) Correlation analysis was conducted to identify the relationship between changes in six glycan structures (specifically binding to PHA-L, SNA, PSA, SBA, RCA120, and AAL) in saliva and disease severity indicators (BP180 antibody titer, BP230 antibody titer, erythema and wheal score, mucosal damage score, BPDAI score). BP patients were categorized into four subgroups based on the positivity of salivary anti-BP180-NC16A and the presence of oral mucosal damage. Spearman’s correlation analysis was used for statistical testing. X indicates p>0.05 after statistical testing. Red color represents positive correlation, while blue color represents negative correlation. The larger and deeper the circle, the stronger the correlation coefficient. The numerical values in the middle represent the Spearman correlation coefficients. (b) Changes in the glycan structures bound to SNA, and AAL in the saliva of BP patients were detected using lectin ELISA before treatment at admission and after significant improvement at discharge (n=11). The Wilcoxon Signed-Rank test was used for statistical analysis. (c) ROC analysis was conducted to assess the glycan structures bound to SNA and AAL in saliva as biomarkers. AUC was used as an indicator to evaluate diagnostic capability.

    Article Snippet: Briefly, during Lectin ELISA of serum and blister fluid, biotinylated lectins were diluted with 1% BSA to the following concentrations: HPA-L (Vector, B-1115) 5μg/mL, SNA (Vector, B-1305) 0.5μg/mL, PSA (Vector, B-1055) 0.5μg/mL, SBA (Vector, B-1015) 0.5μg/mL, RCA120 (Vector, B-1085) 0.05μg/mL, AAL (Vector, B-1395) 0.01μg/mL, while during Lectin ELISA of saliva, biotinylated lectins were diluted to the following concentrations: HPA-L 5μg/mL, SNA 2μg/mL, PSA 1μg/mL, SBA 5μg/mL, RCA120 1μg/mL, AAL 1μg/mL.

    Techniques: Glycoproteomics, Diagnostic Assay, Binding Assay, Enzyme-linked Immunosorbent Assay

    Journal: iScience

    Article Title: Perturbation of placental protein glycosylation by endoplasmic reticulum stress promotes maladaptation of maternal hepatic glucose metabolism

    doi: 10.1016/j.isci.2022.105911

    Figure Lengend Snippet:

    Article Snippet: Pisum Sativum Agglutinin-biotinylated lectin , Vector Laboratories , Cat#B-1055.

    Techniques: Recombinant, Plasmid Preparation, Avidin-Biotin Assay, Lysis, Isolation, Bradford Protein Assay, Labeling, SYBR Green Assay, Mutagenesis, Software